Recombinant Escherichia coli Cell Lysates as a Low-Cost Alternative for Vaccines Against Veterinary Clostridial Diseases

This chapter describes a practical, industry-friendly, and efficient vaccine protocol based on the use of Escherichia coli cell fractions (inclusion bodies or cell lysate supernatant) containing the recombinant antigen. This approach was characterized and evaluated in laboratory and farm animals by...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2411; p. 105
Main Authors Moreira, Jr, Clóvis, Moreira, Gustavo Marçal Schmidt Garcia, Donassolo, Rafael Amaral, Ferreira, Marcos Roberto Alves, Alves, Mariliana Luiza Ferreira, Rodrigues, Rafael Rodrigues, Conceição, Fabricio Rochedo
Format Journal Article
LanguageEnglish
Published United States 2022
Subjects
Animals
Bacterial Vaccines
Escherichia coli - genetics
Escherichia coli - immunology
Escherichia coli Infections - prevention & control
Escherichia coli Infections - veterinary
Escherichia coli Vaccines
Inclusion Bodies
Mice
Vaccines, Synthetic
Cell lysate supernatant
Clostridiosis
Recombinant vaccines
Recombinant antigens
Inclusion bodies
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Summary:This chapter describes a practical, industry-friendly, and efficient vaccine protocol based on the use of Escherichia coli cell fractions (inclusion bodies or cell lysate supernatant) containing the recombinant antigen. This approach was characterized and evaluated in laboratory and farm animals by the seroneutralization assay in mice, thereby showing to be an excellent alternative to induce a protective immune response against clostridial diseases.
ISSN:1940-6029
DOI:10.1007/978-1-0716-1888-2_6