Elicitation of jasmonate-mediated defense responses by mechanical wounding and insect herbivory

Many plant immune responses to biotic stress are mediated by the wound hormone jasmonate (JA). Functional and mechanistic studies of the JA signaling pathway often involve plant manipulations that elicit JA production and subsequent changes in gene expression in local and systemic tissues. Here, we...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1011; p. 51
Main Authors Herde, Marco, Koo, Abraham J K, Howe, Gregg A
Format Journal Article
LanguageEnglish
Published United States 2013
Subjects
Animals
Arabidopsis - immunology
Arabidopsis - metabolism
Butterflies - physiology
Cyclopentanes - metabolism
Disease Resistance
Herbivory
Larva - physiology
Oxylipins - metabolism
Plant Growth Regulators - physiology
Plant Leaves - immunology
Plant Leaves - metabolism
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Summary:Many plant immune responses to biotic stress are mediated by the wound hormone jasmonate (JA). Functional and mechanistic studies of the JA signaling pathway often involve plant manipulations that elicit JA production and subsequent changes in gene expression in local and systemic tissues. Here, we describe a simple mechanical wounding procedure to effectively trigger JA responses in the Arabidopsis thaliana rosette. For comparison, we also present a plant-insect bioassay to elicit defense responses with the chewing insect Trichoplusia ni. This latter procedure can be used to determine the effect of JA-regulated defenses on growth and development of insect herbivores.
ISSN:1940-6029
DOI:10.1007/978-1-62703-414-2_5