Establishment of AIDS Animal Model with SIVmac239 Infected Chinese Rhesus Monkey

• Published in: Virologica Sinica Vol. 22; no. 6; pp. 509 - 516
• Main Authors: Li, Ming-hua, Li, Shao-you, Xia, Hou-jun, Wang, Lu, Wang, Yuan-yuan, Zhang, Gao-hong, Zheng, Yong-tang
• Format: Journal Article
• Language: English
• Published: Laboratory of Molecular Immunopharmacology, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China%Laboratory of Molecular Immunopharmacology, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 01.12.2007; Graduate School of the Chinese Academy of Sciences, Beijing 100049, China
• Subjects: 动物模型; 恒河猴; 艾滋病; 艾滋病病毒; SIV; Chinese rhesus monkey; AIDS; SIVmac239; HIV; Primate animal model
• ISSN: 1674-0769; 1995-820X
• DOI: 10.1007/s12250-007-0053-9

In the present research, two Chinese rhesus monkeys were inoculated intravenously with 5000 TCID50 of SIVmac239. The changes in the numbers of CD4^＋T lymphocyte in peripheral blood, plasma viral loads, proviral DNA and humoral antibodies against virus were periodically monitored during 121 days. At the early stage of infection, proviral DNA had been detected in PBMCs, and infectious SIVmac239 virus had been isolated from PBMCs. At the same period, the numbers of CD4^＋ T lymphocytes were significantly decreased, and maintained at low level during the 121-day period of infection. Plasma viral loads reached the peak at week 2 post-inoculation and kept at a steady state subsequently. Moreover, antibodies against viral proteins were detected from plasma. All the results showed that the two Chinese rhesus monkeys had been infected with SIVmac239 successfully. This animal model can be applied for further AIDS researches.