Gold nanoparticle antibody conjugates for use in competitive lateral flow assays

Gold nanoparticles (GNPs) are widely used in a variety of biomedical diagnostic assays and for imaging. Their popularity stems from key properties such as their low toxicity and high extinction coefficients, as well as straightforward synthesis methods that allow GNPs to be produced quickly and inex...

Full description

Saved in:
Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 906; p. 45
Main Authors Bailes, Julian, Mayoss, Samantha, Teale, Phil, Soloviev, Mikhail
Format Journal Article
LanguageEnglish
Published United States 2012
Subjects
Antibodies - chemistry
Antibodies - immunology
Gold - chemistry
Immunochromatography - methods
Metal Nanoparticles - chemistry
Oxazepam - immunology
Point-of-Care Systems
Online AccessGet more information

Cover

More Information
Summary:Gold nanoparticles (GNPs) are widely used in a variety of biomedical diagnostic assays and for imaging. Their popularity stems from key properties such as their low toxicity and high extinction coefficients, as well as straightforward synthesis methods that allow GNPs to be produced quickly and inexpensively. Here we describe the use of GNPs for visual detection in a lateral flow assay using benzodiazepine affinity assay to illustrate the methods.
ISSN:1940-6029
DOI:10.1007/978-1-61779-953-2_4