Use of Echinoderm Gametes and Early Embryos for Studying Meiosis and Mitosis

The early embryos of sea urchins and other echinoderms have served as experimental models for the study of cell division since the nineteenth century. Their rapid development, optical clarity, and ease of manipulation continue to offer advantages for studying spindle assembly and cytokinesis. In the...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2415; p. 1
Main Authors Pal, Debadrita, Visconti, Florencia, Sepúlveda-Ramírez, Silvia P, Swartz, S Zachary, Shuster, Charles B
Format Journal Article
LanguageEnglish
Published United States 2022
Subjects
Animals
Germ Cells
Meiosis
Microtubules - metabolism
Mitosis
Sea Urchins
Spindle Apparatus - metabolism
Mitosis
Sea star
Confocal microscopy
Meiosis
Microtubule
Sea urchin
Cytokinesis actin
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Summary:The early embryos of sea urchins and other echinoderms have served as experimental models for the study of cell division since the nineteenth century. Their rapid development, optical clarity, and ease of manipulation continue to offer advantages for studying spindle assembly and cytokinesis. In the absence of transgenic lines, alternative strategies must be employed to visualize microtubules and actin. Here, we describe methods to visualize actin and microtubule using either purified, recombinant proteins, or probes in in vitro-transcribed mRNAs.
ISSN:1940-6029
DOI:10.1007/978-1-0716-1904-9_1