Identification of differentially expressed proteins in SH-SY5Y cells treated with resveratrol

• Published in: 中国神经再生研究（英文版） Vol. 6; no. 21; pp. 1612 - 1617
• Main Author: Ying Wang Zhong Dong Hongyan Fan Ming Chang Guoyi Li Linsen Hu
• Format: Journal Article
• Language: English
• Published: Department of Neurology, First Affiliated Hospital, Jilin University, Changchun 130021, Jilin Province, China%Department of Pharmacology, Jilin Medical College of Basic Medicine, Jilin 132011, Jilin Province, China 01.07.2011
• Subjects: T细胞受体; 内质网应激; 基质辅助激光解吸电离飞行时间质谱; 差异表达; 白藜芦醇; 神经保护作用; 蛋白质; 鉴定; two-dimensional difference gel electrophoresis; neural regeneration; proteomics; resveratrol; SH-SY5Y cells
• ISSN: 1673-5374
• DOI: 10.3969/j.issn.1673-5374.2011.21.002

To gain insight into the molecular mechanisms of resveratrol-mediated neuroprotection, two-dimensional difference gel electrophoresis in combination with matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to identify proteins differentially-expressed in SH-SY5Y cells treated with resveratrol. Compared with the control group, resveratrol treatment significantly affected the expression of four proteins： endoplasmic reticulum oxidoreductin 1-like protein alpha, p21-activated kinase 1, Archain 1, and T cell receptor beta chain. The former three were downregulated and the latter was upregulated. These proteins are primarily associated with endoplasmic reticulum stress, intracellular trafficking, and immune function.