Granulocyte colony-stimulating factor promotes growth of processes, growth associated protein 43 and microtubule-associated protein 2 expression in cultured rat retinal ganglion cells in vitro

• Published in: 中国神经再生研究（英文版） Vol. 6; no. 31; pp. 2435 - 2440
• Main Author: Haitao Xu Yuying Jiang Xiuhong Qin Lihui Si Jie Zhao Lijuan Liu Yazhen Wu
• Format: Journal Article
• Language: English
• Published: Department of Ophthalmology, Second Clinical Hospital of Jilin University, Changchun 130041, Jilin Province, China%Department of Ophthalmology, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China%Department of Gynaecology and Obstetrics, Second Clinical Hospital of Jilin University, Changchun 130041, Jilin Province, China%Changchun Medical College, Changchun 130013, Jilin Province, China%Emergency Center of Changchun, Changchun 130021, Jilin Province, China 15.12.2011
• Subjects: mRNA; 体外培养; 大鼠; 微管相关蛋白; 生长相关蛋白; 粒细胞集落刺激因子; 蛋白质含量; 视网膜神经节细胞; axons; growth-associated protein 43; neural regeneration; granulocyte colony-stimulating factor; ganglion cells; microtubule-associated protein 2
• ISSN: 1673-5374
• DOI: 10.3969/j.issn.1673-5374.2011.31.007

Following granulocyte colony-stimulating factor （G-CSF） treatment,the growth of processes in cul-tured rat retinal ganglion cells （RGCs） in vitro,expression of growth associated protein 43,and expression of microtubule-associated protein 2 mRNA expression were significantly increased.In contrast,RhoA/Rock protein content was significantly reduced by G-CSF treatment.These results indicate that G-CSF promotes the growth of processes in RGCs and increases the expression of growth-associated protein 43 and microtubule-associated protein 2 mRNA by inhibiting the RhoA/Rock pathway,thereby benefiting axonal repair in RGCs exposed to hypoxia.