Effect of formalincresol on mouse peritoneal macrophages

• Published in: Japanese Journal of Oral Biology Vol. 29; no. 1; pp. 13 - 24
• Main Authors: Kuroki, Kayoko, Uchiyama, Choji, Ohsumi, Tomoko, Murakami, Yuji
• Format: Journal Article
• Language: English
• Published: Japanese Association for Oral Biology 1987
• Subjects: formalincresol; LPS; morphological change; Mouse peritoneal macrophage; peptone
• ISSN: 0385-0137
• DOI: 10.2330/joralbiosci1965.29.13

When mouse peritoneal macrophages induced by pepton (P-M) and those by lipopolysaccharide (L-M) are cultivated in vitro, both P-M and L-M are classified into three cell types: rounded cells (type A), short spindle-like cells (type B) and long spindle-like or spreading cells (type C). The P-M and L-M cells were exposed to formalincresol (FC), a mixture containing the same quantity each of formalin and cresol, in the concentrations of 5 to 1000μg/ml for 5 to 30 minutes. The effect of FC were observed morphologically, and a comparison was also made between P-M and L-M and among types A, B and C. Both P-M and L-M were nearly intact in the concentration of 5 μg/ml of FC, suffered deformation of cell shrinkage in a concentration of 10μg/ml, and kept a less-changed form and nearly fixed in 100μg/ml. L-M was changed to a lesser extent than P-M by 10μg/ml of FC. Cells of type C showed a tendency to decrease and those of type A to increase with FC. Continuous observation under a phase contrast microscope made it clear that the response to FC of the type C cell was the most sensitive of the three types. Under the scanning electron microscope, frill-like ruffles were observed in type A and foam-like surface protrusions of various sizes in types B and C cells when the P-M were exposed to FC in 10μg/ml. These results indicate (1) that the mechanism of the effect on the macrophages of FC at 10μg/ml is different from that at and over 100μg/ml, (2) that L-M showed a higher tolerance for FC than P-M at concentrations of 100μg/ml and below, and (3) morphological degeneration affected types C, B and A in descending order.