Inositol phosphate formation in fMet-Leu-Phe-stimulated human neutrophils does not require an increase in the cytosolic free Ca2+ concentration

The accumulation of inositol phosphates in myo-[3H]inositol-labelled human neutrophils stimulated with the chemotactic peptide fMet-Leu-Phe was measured. The challenge with the chemotactic peptide caused the generation of inositol monophosphate (InsP), inositol bisphosphate (InsP2) and inositol tris...

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Bibliographic Details
Published inBiochemical journal Vol. 229; no. 2; pp. 361 - 367
Main Authors Di Virgilio, F, Vicentini, L M, Treves, S, Riz, G, Pozzan, T
Format Journal Article
LanguageEnglish
Published England 15.07.1985
Subjects
Calcium - metabolism
Cytoplasm - metabolism
Cytosol - metabolism
Humans
Inositol Phosphates - blood
N-Formylmethionine Leucyl-Phenylalanine - pharmacology
Neutrophils - metabolism
Oxygen Consumption
Sugar Phosphates - blood
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Summary:The accumulation of inositol phosphates in myo-[3H]inositol-labelled human neutrophils stimulated with the chemotactic peptide fMet-Leu-Phe was measured. The challenge with the chemotactic peptide caused the generation of inositol monophosphate (InsP), inositol bisphosphate (InsP2) and inositol trisphosphate (InsP3). The formation of the three inositol phosphates followed a differential time course: InsP3 accumulated very rapidly and transiently, whereas InsP increased steadily for more than 2 min. Inositol phosphate formation was only partially decreased by procedures which prevented the fMet-Leu-Phe-dependent increase of cytosolic free Ca2+ concentration.
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ISSN:0264-6021
1470-8728
DOI:10.1042/bj2290361