Polygenic nature of spontaneous diabetes in the rat. Permissive MHC haplotype and presence of the lymphopenic trait of the BB rat are not sufficient to produce susceptibility
Polygenic nature of spontaneous diabetes in the rat. Permissive MHC haplotype and presence of the lymphopenic trait of the BB rat are not sufficient to produce susceptibility. E Colle , A Fuks , P Poussier , P Edouard and R D Guttmann McGill University-Montreal Childrens Hospital Research Institute,...
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Published in | Diabetes (New York, N.Y.) Vol. 41; no. 12; pp. 1617 - 1623 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
American Diabetes Association
01.12.1992
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Online Access | Get full text |
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Summary: | Polygenic nature of spontaneous diabetes in the rat. Permissive MHC haplotype and presence of the lymphopenic trait of the
BB rat are not sufficient to produce susceptibility.
E Colle ,
A Fuks ,
P Poussier ,
P Edouard and
R D Guttmann
McGill University-Montreal Childrens Hospital Research Institute, Québec, Canada.
Abstract
We describe the phenotypic characteristics of animals in the fifth backcross-intercross generation of a breeding program in
which the RT1 u haplotype and the phenotypic trait responsible for the T-lymphopenia of BB rats have been transferred to the
ACI background. In this generation of animals, 24% were lymphopenic with decreased numbers of PBL expressing CD5, TCR alpha,
and RT6. The PBL of the lymphopenic animals had a decreased mitogenic response to ConA. All of the nonlymphopenic animals
were homozygous for RT6.2. Phenotypic analysis of intestinal IEL revealed that this was also the case for the lymphopenic
animals. Moreover, IEL of the lymphopenic animals exhibited a pattern of staining (increased numbers of TCR alpha beta+CD4+CD8+
and decreased numbers of TCR alpha beta+CD4-CD8+) similar to that of BB DP animals. The ACI.1U(BB)-lymphopenic animals, although
having two of the genetic traits associated with the expression of spontaneous diabetes mellitus, uniformly fail to develop
diabetes. Breeding studies in which these animals were crossed with BB and hBB rats suggest that other genes are necessary
for development of overt diabetes. |
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ISSN: | 0012-1797 1939-327X 0012-1797 |
DOI: | 10.2337/diabetes.41.12.1617 |