Cloning and identification of magnetic field-responsive genes in Daudi cells

In order to study the molecular mechanisms of ELF MF biological effects, the differential display (DD) technology has been used to isolate differentially expressed cDNA fragments in Daudi cells after 50 Hz MF exposure and sham-exposure. Using total RNA isolated from 0.8 mT 50 Hz MF exposed cells and...

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Bibliographic Details
Published inChinese science bulletin Vol. 45; no. 11; pp. 1006 - 1010
Main Authors Wu, Ruiying, Jiang, Huai, Hu, Genlin, Fu, Yiti, Lu, Deqiang
Format Journal Article
LanguageEnglish
Published Beijing Springer Nature B.V 01.06.2000
Subjects
Biological effects
Cloning
Deoxyribonucleic acid
Differential display
DNA
Exposure
Magnetic fields
Molecular modelling
Nucleotide sequence
Sequence analysis
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Summary:In order to study the molecular mechanisms of ELF MF biological effects, the differential display (DD) technology has been used to isolate differentially expressed cDNA fragments in Daudi cells after 50 Hz MF exposure and sham-exposure. Using total RNA isolated from 0.8 mT 50 Hz MF exposed cells and those from sham-exposed cells, one differentially regulated transcript has been found by DD and the expression pattern of the same transcript has been verified by reverse-Northern and Northern analyses. DNA sequence analysis and Genbank research demonstrated that the cDNA fragment (MF-CA) is a novel gene in human cell which is induced by ELF MF.
ISSN:1001-6538
2095-9273
1861-9541
2095-9281
DOI:10.1007/BF02884981