Induction of manganese peroxidase and laccase by Lentinula edodes under liquid culture conditions and their isozyme detection by enzymatic staining on native-PAGE

The white-rot basidomycete Lentinula edodes often produces the lignin-degrading enzymes manganese peroxidase (MnP; EC 1.11.1.13) and laccase (Lcc; EC 1.10.3.2) in sawdust-based media. In the present study, MnP from L. edodes was induced under liquid culture supplemented with sawdust extracts of Cast...

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Bibliographic Details
Published inMycoscience Vol. 52; no. 2; pp. 132 - 136
Main Authors Saeki, Naomi, Takeda, Hironori, Tanesaka, Eiji, Yoshida, Motonobu
Format Journal Article
LanguageEnglish
Published The Mycological Society of Japan 2011
Subjects
Breeding
Copper ion
Extracellular enzyme
Phenoloxidases
Shiitake mushroom
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Summary:The white-rot basidomycete Lentinula edodes often produces the lignin-degrading enzymes manganese peroxidase (MnP; EC 1.11.1.13) and laccase (Lcc; EC 1.10.3.2) in sawdust-based media. In the present study, MnP from L. edodes was induced under liquid culture supplemented with sawdust extracts of Castanopsis cuspidata. Lcc activity was induced by the addition of 2 mM CuSO4 5H2O into the same media 7 days after initial inoculation. Phenoloxidase enzymes were distinguished by polyacrylamide gel electrophoresis (native- PAGE), followed by sequential enzymatic staining with an improved staining solution. The isozyme bands detected under MnP-induced conditions were identified as manganese peroxidase (lemnp2) and bands detected under Lcc-induced conditions were identified as laccase (lcc1) by Q-TOF mass spectrometry.
ISSN:1340-3540
1618-2545
DOI:10.47371/S10267-010-0076-1