Comparative evaluation of sensitivity and specificity of immunochromatography kit for the rapid detection of norovirus and rotavirus in Bangladesh [version 1; peer review: 2 approved with reservations]

• Published in: F1000 research Vol. 8; p. 173
• Main Authors: Shaha, Modhusudon, Sifat, Sadia Farzana, Mamun, Md. Al, Billah, Md. Baki, Sharif, Nadim, Nobel, Nasir Uddin, Parvez, Anowar Khasru, Talukder, Ali Azam, Nomura, Akiko, Ushijima, Hiroshi, Dey, Shuvra Kanti
• Format: Journal Article
• Language: English
• Published: 2019
• Subjects: Immunochromatography kit; Bangladesh; Norovirus; Rotavirus; Rapid detection
• ISSN: 2046-1402; 2046-1402
• DOI: 10.12688/f1000research.17362.1

We report a comprehensive analysis of sensitivity and specificity of immunochromatography kit (IC Kit) for the rapid detection of norovirus and rotavirus in Bangladesh. The IC kit (IP-Noro/Rota) provides highest sensitivity (100%) to both viruses compared to the reference method reverse transcription- polymerase chain reaction (RT- PCR) for diagnosis. Furthermore, the test provides a high specificity of 98.9% and 96.1% to diagnose norovirus and rotavirus, respectively, as well as good agreement with the reference method. We also found high prevalence of rotavirus infection (74%) among Bangladeshi pediatric population, of which most of the patients were less than five years old, suffering from severe dehydration, abdominal pain and vomiting. This study is the first to report the ease and rapid detection of norovirus and rotavirus by IC kits in Bangladesh. Therefore, IP-Noro/Rota kit is recommended for the rapid detection of these viruses in routine diagnosis as well as during outbreaks.