Isolation and characterization of novel marine luminescent bacteria from Diu beach, India

• Published in: Journal of pharmacy research Vol. 7; no. 6; pp. 529 - 533
• Main Authors: Shanware, Arti, Thakre, Neha, Pande, Sunil
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2013
• Subjects: 16S rRNA gene; Bioluminescence; Biosensors; Diu beach; Vibrio rotiferianus; Bioluminescence; Diu beach; Vibrio rotiferianus; Biosensors; 16S rRNA gene
• ISSN: 0974-6943; 0974-6943
• DOI: 10.1016/j.jopr.2013.05.019

To carry out the isolation and characterization of novel marine luminescent bacteria from Diu beach, India. In the present study attempt has been made to isolate luminescent bacterial species from marine water sampled from Diu beach, India, using standard plating method on nutrient medium. Obtained isolate was analyzed susceptibility against antibiotics and heavy metals in order to use them in bio-sensing system. Isolated bacterium was amplified for 16S rRNA gene by PCR and further identified by aligning through BLAST and MEGA software. The Vibrio rotiferianus when growing prominently at 22 °C shown tolerance to low concentration of heavy metals & resistance to few antibiotics which makes isolate, a potential bio-sensing agent. Further, molecular characterization including 16S rRNA gene and its phylogenetic analysis confirmed that the isolated strain as V. rotiferianus. The use of pharmaceuticals products is on the rise and in turn its pollution is rising in an ecosystem. The isolated strain may act as a potential biosensing agent as it responded to change in luminescence for even nanomolar quantities of several heavy metals/antibiotics. In conclusion, study highlighted V. rotiferianus isolated from Diu beach could be utilized in detection and quantitative estimation of pollutant by change in luminescence level of V. rotiferianus.