Syndecan-2 overexpression regulates adhesion and migration through cooperation with integrin α2

• Published in: Biochemical and biophysical research communications Vol. 384; no. 2; pp. 231 - 235
• Main Authors: Choi, Sojoong, Kim, Yeonhee, Park, Haein, Han, Inn-Oc, Chung, Eunkyung, Lee, Sung-Yul, Kim, Yong-Bae, Lee, Jung Weon, Oh, Eok-Soo, Yi, Jae Youn
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 26.06.2009
• Subjects: Adhesion; Colon cancer cell; Integrin α2; Invasion; Migration; Syndecan-2; Colon cancer cell; Integrin α2; Adhesion; Migration; Syndecan-2; Invasion
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2009.04.093

Syndecan-2, a transmembrane heparan sulfate proteoglycan, is known to serve as an adhesion receptor, but details of the regulatory mechanism governing syndecan-2 cell adhesion and migration remain unclear. Here, we examined this regulatory mechanism, showing that overexpression of syndecan-2 enhanced collagen adhesion, cell migration and invasion of normal rat intestinal epithelial cells (RIE1), and increased integrin α2 expression levels. Interestingly, RIE1 cells transfected with either syndecan-2 or integrin α2 showed similar adhesion and migration patterns, and a function-blocking anti-integrin α2 antibody abolished syndecan-2-mediated adhesion and migration. Consistent with these findings, transfection of integrin α2 siRNA diminished syndecan-2-induced cell migration in HCT116 human colon cancer cells. Taken together, these results demonstrate a novel cooperation between syndecan-2 and integrin α2β1 in adhesion-mediated cell migration and invasion. This interactive dynamic might be a possible mechanism underlying the tumorigenic activities of colon cancer cells.