Analytical study of kallikrein and kallikrein-like esterase activity in subfractions from rat kidney cortex microsomes and isolated subcellular membranes

Heavy and light microsomal fractions were subfractioned using high performance zonal rotors, and assayed for apoprotenin sensitive kallikrein-like amidolytic activity (pH 8.2). The activity profiles for the various substrates assayed show rather complex distribution pattern demonstrating kallikrein-...

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Bibliographic Details
Published inAdvances in experimental medicine and biology Vol. 198 Pt A; p. 355
Main Authors Andersen, K J, Ofstad, J
Format Journal Article
LanguageEnglish
Published United States 1986
Subjects
Aminopeptidases - metabolism
Animals
Cell Fractionation
Intracellular Membranes - enzymology
Kallikreins - metabolism
Kidney Cortex - enzymology
Male
Microsomes - enzymology
Rats
Rats, Inbred Strains
Substrate Specificity
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Summary:Heavy and light microsomal fractions were subfractioned using high performance zonal rotors, and assayed for apoprotenin sensitive kallikrein-like amidolytic activity (pH 8.2). The activity profiles for the various substrates assayed show rather complex distribution pattern demonstrating kallikrein-like amidolytic activity in plasma membranes, basolateral membranes, rough endoplasmic reticulum and membranes derived from the Golgi complex.
ISSN:0065-2598
DOI:10.1007/978-1-4684-5143-6_48