A multiplex PCR approach to simultaneously genotype potato towards the resistance alleles Ry-f sto and Ns

A simple and robust multiplex PCR approach was developed for detection of the alleles Ry-fsto and Ns conferring resistance of potato to Potato Virus Y (PVY) and Potato Virus S (PVS), respectively. Cleaved amplified polymorphic sequence (CAPS) markers GP122564 linked to Ry-fsto and SC811260 linked to...

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Published inMolecular breeding Vol. 18; no. 3; pp. 273 - 275
Main Authors Witek, Kamil, Strzelczyk-Żyta, Danuta, Hennig, Jacek, Marczewski, Waldemar
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.10.2006
Subjects
Alleles
Cultivars
Disease resistance
Genetics
genotype
Genotypes
Marker-assisted selection
Molecular biology
Multiplexing
Pathogens
Plant biology
polymerase chain reaction
Potato virus S
Potato virus Y
Potatoes
Vegetables
Viruses
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Summary:A simple and robust multiplex PCR approach was developed for detection of the alleles Ry-fsto and Ns conferring resistance of potato to Potato Virus Y (PVY) and Potato Virus S (PVS), respectively. Cleaved amplified polymorphic sequence (CAPS) markers GP122564 linked to Ry-fsto and SC811260 linked to Ns were amplified in one PCR reaction and identified after simultaneous digestion of the amplicons with restriction enzymes EcoRV and MboI. Effectiveness of this procedure for marker-assisted selection was confirmed in 55 potato cultivars.
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ISSN:1380-3743
1572-9788
DOI:10.1007/s11032-006-9021-6