Oxidation of low-density lipoprotein by Cu2+ and lipoxygenase: an electron spin resonance study

The aim of this work was to obtain spectroscopic evidence for free radicals formed during copper ion- and lipoxygenase-catalyzed oxidation of the low-density lipoprotein. During the initial oxidation phase, a free-radical metabolite derived from the endogenous alpha-tocopherol present in the low-den...

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Bibliographic Details
Published inBiochimica et biophysica acta Vol. 1035; no. 3; pp. 286 - 292
Main Authors Kalyanaraman, B., Antholine, William E., Parthasarathy, Sampath
Format Journal Article
LanguageEnglish
Published Netherlands 14.09.1990
Subjects
Copper - metabolism
Copper - pharmacology
Electron Spin Resonance Spectroscopy
Free Radicals
Humans
Kinetics
Lipid Peroxidation
Lipoproteins, LDL - blood
Lipoxygenase - metabolism
Models, Biological
Protein Binding
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Summary:The aim of this work was to obtain spectroscopic evidence for free radicals formed during copper ion- and lipoxygenase-catalyzed oxidation of the low-density lipoprotein. During the initial oxidation phase, a free-radical metabolite derived from the endogenous alpha-tocopherol present in the low-density lipoprotein was detected by the electron spin resonance technique. The divalent copper ions were bound to the residual EDTA present in the low-density lipoprotein and to the protein. Production of the alpha-tocopherol radical was suppressed in the presence of spin traps. Evidence for the low-density lipoprotein-lipid derived radicals was obtained by ESR-spin trapping methods. Implications of these findings in the oxidative modification of the low-density lipoprotein are discussed.
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ISSN:0304-4165
0006-3002
DOI:10.1016/0304-4165(90)90090-J