Dual-colored bacterial biosensor responsive to cadmium, mercury, and lead for detecting heavy metal pollution in seawater

• Published in: Ecological indicators Vol. 166; p. 112244
• Main Authors: Hu, Shun-yu, Hui, Chang-ye, Wu, Can, Gao, Chao-xian, Huang, Zhenlie, Guo, Yan
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.09.2024; Elsevier
• Subjects: Concurrent detection; Seawater analysis; Toxic metals; Visual detection; Concurrent detection; Seawater analysis; Visual detection; Toxic metals
• ISSN: 1470-160X
• DOI: 10.1016/j.ecolind.2024.112244

[Display omitted] •Detects Cd, Hg, Pb via distinct color changes.•Unprecedented sensitivity: 9.7 nM Cd, 24.4 nM Pb, 0.5 nM Hg.•High salinity tolerance, no sample pre-treatment required.•Synthetic biology for practical, cost-effective environmental monitoring. Estimating bioavailable heavy metals in seawater is closely related to the indication of ecological risk. This study combined a CadR-regulated vioABE expression module and a MerR-regulated VioC expression module to generate a novel dual-colored bacterial cell-based biosensor. After genetic optimization, the preferred biosensor could detect as low as 9.7 nM Cd(II), 24.4 nM Pb(II), and 0.5 nM Hg(II). Increased grey-green intensities were observed in Cd(II) (4.9 nM to 40 μM) and Pb(II) (24.4 nM to 200 μM) exposure groups. Interestingly, increased purple intensity was observed in the Hg(II) exposure group (3.7 to 468.8 nM) in a dose-dependent manner. A low-cost and mini-equipment biosensing process was established for detecting pollutant heavy metals in seawater, with the added advantage of providing information on the bioavailability and cytotoxicity of heavy metals. High salinity weakly interferes with the biosensing response to Hg(II). This study shows that novel colorimetric biosensors have the potential to simultaneously report various toxic metals in environmental water samples, contributing to protecting marine ecology.