Characterization of kininogen deficiency of Brown Norway rat mutant Katholiek strain

A deficiency in the plasma kallikrein-kinin system of Brown Norway rat mutant Katholiek strain (B/N-Ka), first reported by Damas et al. was further characterized. The prolonged activated partial thromboplastin time (APTT) of B/N-Ka rat plasma was corrected by an addition of rat HMW-kininogen, indica...

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Bibliographic Details
Published inAdvances in experimental medicine and biology Vol. 198 Pt A; p. 77
Main Authors Oh-ishi, S, Hayashi, I, Ino, T, Kato, H, Iwanaga, S, Nakano, T
Format Journal Article
LanguageEnglish
Published United States 1986
Subjects
Animals
Female
Guinea Pigs
Immunodiffusion
Kinetics
Kininogens - blood
Kininogens - genetics
Kinins - blood
Male
Mutation
Rats
Rats, Inbred Strains
Rats, Mutant Strains
Species Specificity
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Summary:A deficiency in the plasma kallikrein-kinin system of Brown Norway rat mutant Katholiek strain (B/N-Ka), first reported by Damas et al. was further characterized. The prolonged activated partial thromboplastin time (APTT) of B/N-Ka rat plasma was corrected by an addition of rat HMW-kininogen, indicating B/N-Ka rat is deficient in HMW kininogen. The study of kinin-release of plasmas of the three strains of rat (B/N-Ka, B/N Ki and SD) by several kininogenases expressed that B/N-Ka rat is deficient in LMW kininogen, in addition to HMW kininogen deficiency. The plasmas of the three strains of rat were gel-filtered through Sephacryl S-200 gel and profiles of kinin-release of the fractions were examined by several kininogenases. The result demonstrated that normal rat plasma contains three kinds of kininogen (HMW and LMW kininogens, and T-kininogen), and B/N-Ka plasma contains only T-kininogen. B/N-Ka rat plasma demonstrated T-kininogen antigen but no HMW kininogen by the study of immunodiffusion using their antisera raised in rabbits.
ISSN:0065-2598
DOI:10.1007/978-1-4684-5143-6_11