Simultaneous Determination of Four Substances in Plasma and Dermal Microdialysates of Guinea Pig after Different Acupoints Administration of Fufang Baijiezi Gel

• Published in: Chinese herbal medicines Vol. 7; no. 4; pp. 365 - 370
• Main Authors: Wang, Guo-hua, Zhang, Bao-xian, Nie, Qi-xia, Zang, Chen, Zhu, Qiong
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2015
• Subjects: acupoints administration; Fufang Baijiezi Gel; LC-MS; microdialysis; pharmacokinetics; 同时测定; 复方; 物质; 皮肤; 穴位; 给药; 豚鼠; 透析液; pharmacokinetics; acupoints administration; LC-MS; Fufang Baijiezi Gel; microdialysis
• ISSN: 1674-6384; 2589-3610
• DOI: 10.1016/S1674-6384(15)60065-X

Objective To study the pharmacokinetic properties of Fufang Baijiezi Gel（FBG） after different acupoints administration. Methods Sinapine thiocyanate, tetrahydropalmatine, 6-gingerol, and asarinin, which were four substances of FBG, were determined by a sensitive liquid chromatography tandem mass spectrometry method（LC-MS） both in plasma and dermal microdialysates of guinea pig simultaneously. Microdialysates were separated on an Ultimate？ XB-Phenyl analytical column（150 mm ×2.1 mm, 5μm） and detected by electrospray ionization（ESI） in selected ion monitoring（SIM） mode. The method was validated in terms of selectivity, linearity, sensitivity, and recovery. Result A significant difference was observed in main pharmacokinetic parameters of C max, t max, and AUC between acupoints administration and nonacupoints administration. Conclusion Acupoints administration resulted in a more obvious increase in bioavailability of sinapine thiocyanate, tetrahydropalmatine, 6-gingerol, and asarinin than nonacupoints administration.