Regeneration of Carthamus tinctorius from Jimsar

Objective To investigate the factors significantly affecting the shoot buds regeneration of Carthamus tinctorius from Jimsar （CTJ）. Methods Through tissue culture experiments, the influences of culture temperature, illuminance, relative humidity, explants type, seedlings age, and plant growth regula...

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Bibliographic Details
Published inChinese herbal medicines Vol. 6; no. 3; pp. 237 - 241
Main Authors Fan, Li-jiao, Guo, Mei-li
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.08.2014
Subjects
Carthamus tinctorius
Compositae
MS培养基
regeneration
safflower
tissue culture
培养温度
外植体类型
植物生长调节剂
相对湿度
红花品种
组织培养
芽再生
Carthamus tinctorius
Compositae
safflower
regeneration
tissue culture
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Summary:Objective To investigate the factors significantly affecting the shoot buds regeneration of Carthamus tinctorius from Jimsar （CTJ）. Methods Through tissue culture experiments, the influences of culture temperature, illuminance, relative humidity, explants type, seedlings age, and plant growth regulators added in culture media on in vitro regeneration of CTJ were investigated. Results The culture temperature was set at 24 ℃ in daytime and 16 ℃ at night, illuminance at 9000 lx, and 60% relative humidity were suitable for CTJ regeneration. Cotyledons excised from 6-8 d old seedlings were more responsive than even-aged euphyll, hypocotyl, and root explants because of inducing adventitious buds. The highest percentage of regenerated shoots （79. 1%） with about five adventitious buds per responding explant was obtained from MS basal medium containing 12.0 mg/LTDZ, 2.5 mg/L IBA, and 1.5 mg/L 2-ip （No. 14 medium）. Regenerated shoot buds （80%） could elongate successfully after 1-2 weeks transferred to shoot elongation medium. Conclusion Suitable factors of shoot buds regeneration for CTJ were determined. It may also make a useful reference for regeneration researches of other C. tinctorius varieties in China.
Bibliography:12-1410/R
Objective To investigate the factors significantly affecting the shoot buds regeneration of Carthamus tinctorius from Jimsar （CTJ）. Methods Through tissue culture experiments, the influences of culture temperature, illuminance, relative humidity, explants type, seedlings age, and plant growth regulators added in culture media on in vitro regeneration of CTJ were investigated. Results The culture temperature was set at 24 ℃ in daytime and 16 ℃ at night, illuminance at 9000 lx, and 60% relative humidity were suitable for CTJ regeneration. Cotyledons excised from 6-8 d old seedlings were more responsive than even-aged euphyll, hypocotyl, and root explants because of inducing adventitious buds. The highest percentage of regenerated shoots （79. 1%） with about five adventitious buds per responding explant was obtained from MS basal medium containing 12.0 mg/LTDZ, 2.5 mg/L IBA, and 1.5 mg/L 2-ip （No. 14 medium）. Regenerated shoot buds （80%） could elongate successfully after 1-2 weeks transferred to shoot elongation medium. Conclusion Suitable factors of shoot buds regeneration for CTJ were determined. It may also make a useful reference for regeneration researches of other C. tinctorius varieties in China.
Carthamus tinctorius; Compositae; regeneration; safflower; tissue culture
ISSN:1674-6384
2589-3610
DOI:10.1016/S1674-6384(14)60034-4