Oxidative Modification of Rat Blood Proteins after Lesioning of Capsaicin-Sensitive Nerves and Changes in Nitric Oxide Levels

• Published in: Neuroscience and behavioral physiology Vol. 41; no. 5; pp. 479 - 483
• Main Authors: Tolochko, Z. S., Spiridonov, V. K.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.06.2011; Springer Nature B.V
• Subjects: Behavioral Sciences; Biomedical and Life Sciences; Biomedicine; Metabolism; Neurobiology; Neurosciences; Nitric oxide; Physiology; Proteins; active forms of oxygen; oxidative destruction of proteins; nitric oxide; capsaicin-sensitive nerves
• ISSN: 0097-0549; 1573-899X
• DOI: 10.1007/s11055-011-9440-7

Oxidative modification of blood proteins in rats after lesioning of afferent nerves with capsaicin (150 mg/kg, s.c.) and changes in nitric oxide (NO) contents were studied in terms of levels of protein carbonyl derivatives. Deafferentation of capsaicin-sensitive nerves induced increases in the level of protein carbonyl derivatives. Increases in NO levels using L-arginine did not alter measures of the oxidative modification of proteins after lesioning of capsaicin-sensitive innervation. A similar lack of effect was seen after blockade of neuronal NO synthase (n-NOS) by the selective inhibitor 7-nitroindazole (7-NI). Increases in markers of oxidative destruction of proteins in capsaicin-treated animals were seen after blockade of NO synthesis by the nonselective inhibitor L-NAME. These results show that deafferentation of capsaicin-sensitive nerves induces oxidative modification of proteins and that this process involves NO.