Structure and development of the stigma, style and ovary of Caesalpinia pulcherrima (L.) Sw., post-anthesis, pre- and post-pollination

• Published in: Botanical journal of the Linnean Society Vol. 118; no. 4; pp. 275 - 288
• Main Authors: OWENS, S. J., PRYCHID, C., COX, A. V.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.08.1995
• Subjects: pollen germination; pollen tube growth; transmitting tissue
• ISSN: 0024-4074; 1095-8339
• DOI: 10.1111/j.1095-8339.1995.tb00472.x

The stigma of Caesalpinia pulcherrima is crateriform. The crater continues as a slit‐like canal through the style and into the ovary. Both crater and canal are lined by several layers of fusiform and thin‐walled cells which are continuous in two narrow regions in the ovary. Postanthesis and before pollination, the middle lamella of cells lining the stigmatic crater and stylar transmitting tissue undergoes dissolution. This occurs in a progression down the style with cells separating partially or wholly from neighbours. Dissolution is initiated at intercellular junctions where wall fibrils loosen and variously‐sized and ‐shaped holes appear. Cytoplasmic changes include increased dictyosome activity, increased rough and smooth endoplasmic reticulum at the periphery of cells and accumulation of electron opaque deposits at the plasma membrane. The crater fills with stigmatic fluid and the diameter of the stylar canal increases. Pollen germinates in the secretion‐filled crater, and pollen tubes grow down the style between the cells of the transmitting tissue but do not enter the canal. They emerge at the entrance to the ovary cavity and grow over one or two narrow strips of ovarian transmitting tissue cells which are present throughout the length of the ovary close to the ovules. This ensures that tubes grow in close proximity to the micropyles.