Purification and characterization of strawberry crinkle virus

• Published in: Phytopathology Vol. 80; no. 3; pp. 282 - 287
• Main Authors: Hunter, B.G. (University of California, Berkeley), Richardson, J, Dietzgen, R.G, Sylvester, E.S, Jackson, A.O, Morris, T.J
• Format: Journal Article
• Language: English
• Published: St. Paul, MN American Phytopathological Society 01.03.1990
• Subjects: AISLAMIENTO (TECNICA); Biological and medical sciences; ELECTROFORESIS; ELECTROPHORESE; EPURATION; FRAGARIA; Fundamental and applied biological sciences. Psychology; GLICOPROTEINAS; GLYCOPROTEINE; IMMUNOLOGIE; INMUNOLOGIA; ISOLEMENT; PESO MOLECULAR; PHYSALIS; Phytopathology. Animal pests. Plant and forest protection; Plant viruses and viroids; POIDS MOLECULAIRE; PURIFICACION; SEPARACION; SEPARATION; Systematics. Structure, properties and multiplication. Genetics; VIRUS DE LAS PLANTAS; VIRUS DES VEGETAUX; Small fruits; Stawberry crinkle virus; Rosaceae; Phytopathogen; Characterization; Binding protein; Rhabdoviridae; Dicotyledones; Host plant; Angiospermae; Solanaceae; Structure; Filtration; Fragaria; Purification; Antibody; Gel electrophoresis; Physalis; Electron microscopy; Sedimentation; Virus; Density gradient centrifugation; Analysis method; Fruit crop; Spermatophyta
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/Phyto-80-282

Strawberry crinkle virus (SCV), a cytoplasmic rhabdovirus, has proven difficult to purify from strawberry leaves. By testing alternative hosts and different procedures, we have succeeded in purifying SCV from leaves of Physalis floridana using a celite filtration method. Two classes of particles differing in sedimentation rate could be distinguished by sucrose density gradient centrifugation. Electron microscopic examination of these purified preparations revealed the presence of bacilliform particles and an apparent size difference between the two classes. Polyacrylamide gel electrophoresis of sodium dodecyl sulfate-disrupted virions showed that SCV contains three major structural proteins with molecular weights estimated to be 78, 47, and 25 kDa. The 78-kDa protein bound lectins, identifying it as the viral glycoprotein. The SCV protein profile resembles that of lettuce necrotic yellows virus (LNYV) but differs appreciably from sonchus yellow net virus (SYNV). SCV has no detectable serological relatedness to LNYV or SYNV. These results suggest that SCV is distinct from other previously characterized rhabdoviruses