Catharanthus roseus-mediated CuAl2O4 nanocomposites for evaluation of killing kinetics

• Published in: Chemosphere (Oxford) Vol. 359; p. 142369
• Main Authors: Potbhare, Ajay K., Yerpude, Sachin, Daddemal-Chaudhary, Ankita R., Lambat, Ashish, Mondal, Aniruddha, Dadure, Kanhaiya M., Rai, Alok R., Abdala, Ahmed, Chaudhary, Ratiram G.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.07.2024
• Subjects: Antibacterial assay; Bioinspired CuAl2O4 NCs; Catharanthus roseus; Killing kinetics; Molecular modulation; Antibacterial assay; Catharanthus roseus; Bioinspired CuAl2O4 NCs; Molecular modulation; Killing kinetics
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2024.142369

The present article portrayed on the killing kinetic of human pathogenic bacteria using bioinspired mesoporous CuAl2O4 nanocomposites (NCs). The NCs was fabricated using leaf extract of medicinal plant Catharanthus roseus (CR) as a green reducer and stabilizer. As bio-fabricated material was calcined at 800 °C and characterized by several analytical techniques like X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), Ultraviolet–Visible Diffuse Reflectance Spectroscopy (UV-DRS), Energy Dispersive X-Ray Spectroscopy (EDS), X-Ray Photoelectron Spectroscopy (XPS), Raman, Brunauer-Emmett-Teller (BET), Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM) to authenticate its structure, phase, chemical bonding, chemical state, size and morphology behaviors. XRD and TEM revealed a reduced crystallite and nanoscale sizes of biosynthesized NCs. Moreover, XRD study exposed a cubic-structure of material, while transmission electron microscopy rendered an average particles size in range 10–15 nm. However, BET profile advocates a mesoporous nature of the particles. An effective biological molecular docking modulation assessed by substituting natural inhibitor by bioinspired NCs, while the protein PDB ID 4Z8D FabH as a receptor site for the present investigation. After assessment of molecular docking examination, the antibacterial activity of bioinspired NCs were performed against Staphylococcus aureus, Bacillus subtillis, Klebsiella pneumoniae and Escherichia coli using agar-well method. The broth culture method was employed on different pathogenic strains by kinetic growth assays and colony forming unit. [Display omitted] •Bioinspired mesoporous nature of Catharanthus roseus-mediated CuAl2O4 nanocomposites.•The molecular modulation revealed outstanding docking energies −9.589 to −11.2456 kcal mol−1 for non-GA and GA (PDB ID 4Z8D FabH).•The killing kinetics study revealed, a significant growth decline after 4–12 h against gram-negative and gram-positive bacteria.•An admirable and outstanding cytotoxicity was observed for E. coli and K. pneumoniae.•The gram-positive bacteria show initial growth at 2–4 h, while growth declines after 4–12 h.