Isolation, characterization, and metabolism of the glycated and nonglycated subfractions of low-density lipoproteins isolated from type I diabetic patients and nondiabetic subjects
Isolation, characterization, and metabolism of the glycated and nonglycated subfractions of low-density lipoproteins isolated from type I diabetic patients and nondiabetic subjects. R L Klein , M Laimins and M F Lopes-Virella Ralph H. Johnson Department of Veterans Affairs Medical Center, Charleston...
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Published in | Diabetes (New York, N.Y.) Vol. 44; no. 9; pp. 1093 - 1098 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
American Diabetes Association
01.09.1995
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Online Access | Get full text |
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Summary: | Isolation, characterization, and metabolism of the glycated and nonglycated subfractions of low-density lipoproteins isolated
from type I diabetic patients and nondiabetic subjects.
R L Klein ,
M Laimins and
M F Lopes-Virella
Ralph H. Johnson Department of Veterans Affairs Medical Center, Charleston, South Carolina 29401, USA.
Abstract
The total low-density lipoprotein (LDL) fraction was isolated from 21 patients with type I diabetes and 7 nondiabetic normolipemic
subjects. The LDL was separated into two subfractions, one glycated (G-LDL) and one nonglycated (N-LDL), using affinity chromatography.
G-LDL comprised 21.1 +/- 3.6 and 5.2 +/- 0.6% of the total LDL in diabetic patients and normal subjects, respectively. G-LDL
isolated from both diabetic patients and normal subjects was significantly more glycated than N-LDL isolated from the same
subject. G-LDL isolated from both diabetic patients and normal subjects was enriched in triglycerides. The metabolism of N-LDL
and G-LDL was investigated in human fibroblasts, which express only the classical LDL receptor, and in human monocyte-derived
macrophages, which also express a receptor for G-LDL. In fibroblasts, the rates of receptor-mediated accumulation of N-LDL
isolated from normal subjects and diabetic patients were significantly greater (P < 0.01) than those of G-LDL. In contrast,
when the same LDL subfractions were incubated with human monocyte-derived macrophages, the rates of receptor-mediated accumulation
of G-LDL isolated from both groups were significantly greater (P < 0.01) than those of N-LDL. Rates of degradation of G-LDL
by human macrophages were not significantly different from those of N-LDL during short-term incubations but reached statistical
significance (P < 0.05) when LDL subfractions were incubated with cells for 24 h. |
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ISSN: | 0012-1797 1939-327X 0012-1797 |
DOI: | 10.2337/diabetes.44.9.1093 |