ERRATUM: Kidney decontamination during perfusion for transplantation procedure: In vitro and ex vivo viability analysis

• Published in: Journal of biophotonics Vol. 16; no. 2; pp. e202200363 - n/a
• Main Authors: Goenaga‐Mafud, Loraine C., Gamez, Yordania Matos, Campos, Carolina P., Vollet‐Filho, Jose D., Inada, Natalia Mayumi, Kurachi, Cristina, Bagnato, Vanderlei Salvador
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY‐VCH Verlag GmbH & Co. KGaA 01.02.2023; Wiley Subscription Services, Inc
• Subjects: bacterial inactivation; custodiol; kidney decontamination; kidney transplantation; ultraviolet‐c radiation; kidney decontamination; custodiol; bacterial inactivation; kidney transplantation; ultraviolet-c radiation
• ISSN: 1864-063X; 1864-0648
• DOI: 10.1002/jbio.202200363

Organ transplantations have an increasing medical relevance. It is becoming a regular procedure with an increase in individuals waiting for organs. The increase in the number of discarded organs is mostly due to the donor's bacterial and/or viral infection. In this article, we are demonstrating the feasibility of reduction of the bacterial load in the kidney model by using Ultraviolet‐C (UV‐C) as a germicidal agent in circulating liquids. Using Staphylococcus aureus as a bacteria model, we were able to demonstrate that in less than 30 min of liquid circulation and associated to irradiation, the bacterial load of the perfusate Custodiol® HTK, histidine‐tryptophan‐ketoglutarate (solution with 5 log CFU ml−1), was fully eliminated. A modeling approach was created to verify the possibility of bacterial load decrease, when an organ (here, a renal experimental model) is present in the circuit, releasing a varied rate of microorganisms over time, while the solution is irradiated. Finally, we use an ex vivo model with a swine kidney, circulating in the preservation solution with a Lifeport® Kidney Transporter machine, to demonstrate that we can contaminate the organ and then promote the elimination of the microbiological load. The results show the feasibility of the technique. The graphical generally represents the objective of the work, presenting a sequence of experiments as are the final ex vivo studies of the article. Initially, it shows the organ extracted from the pig being perfused in the perfusion machine with contaminated HTK and at the same time this liquid is decontaminated by the UV‐C decontamination system, obtaining results after plating the collected samples to verify bacterial growth.