Flow cytometry analysis of epithelial cell populations from touch samples using the BD Influx flow cytometry platform [version 1; peer review: 1 approved with reservations, 1 not approved]

'Touch' or trace cell mixtures submitted as evidence are a significant problem for forensic laboratories as they can render resulting genetic profiles difficult or even impossible to interpret. Optical signatures that distinguish epidermal cell populations from different contributors could...

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Bibliographic Details
Published inF1000 research Vol. 5; p. 390
Main Authors Kwon, Ye Jin, Stanciu, Cristina E, Philpott, M. Katherine, Ehrhardt, Christopher J
Format Journal Article
LanguageEnglish
Published 2016
Subjects
Experimental Biophysical Methods
Genomics
forensic science
flow cytometry
epithelial cell
touch mixtures
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Summary:'Touch' or trace cell mixtures submitted as evidence are a significant problem for forensic laboratories as they can render resulting genetic profiles difficult or even impossible to interpret. Optical signatures that distinguish epidermal cell populations from different contributors could facilitate the physical separation of mixture components prior to genetic analysis, and potentially the downstream production of single source profiles and/or simplified mixtures.  For this dataset, optical properties including forwards scatter (FSC), side scatter (SSC), and fluorescence emissions in the Allophycocyanin (APC) channel were measured in epithelial cell populations from touch samples collected from several different contributors on multiple days to assess inter- and intra-contributor variability.
ISSN:2046-1402
2046-1402
DOI:10.12688/f1000research.8338.1