Activation of alveolar epithelial ER stress by β-coronavirus infection disrupts surfactant homeostasis in mice: implications for COVID-19 respiratory failure

• Published in: American journal of physiology. Lung cellular and molecular physiology Vol. 327; no. 2; pp. L232 - L249
• Main Authors: Murthy, Aditi, Rodriguez, Luis R, Dimopoulos, Thalia, Bui, Sarah, Iyer, Swati, Chavez, Katrina, Tomer, Yaniv, Abraham, Valsamma, Cooper, Charlotte, Renner, David M, Katzen, Jeremy B, Bentley, Ian D, Ghadiali, Samir N, Englert, Joshua A, Weiss, Susan R, Beers, Michael F
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.08.2024
• Subjects: Alveolar Epithelial Cells - metabolism; Alveolar Epithelial Cells - pathology; Alveolar Epithelial Cells - virology; Alveoli; Animal models; Animals; Betacoronavirus; Bronchus; Cell activation; Cell culture; Cellular stress response; Coronavirus Infections - complications; Coronavirus Infections - metabolism; Coronavirus Infections - pathology; Coronavirus Infections - virology; Coronaviruses; COVID-19; COVID-19 - complications; COVID-19 - metabolism; COVID-19 - pathology; COVID-19 - virology; Disease Models, Animal; eIF-2 Kinase - metabolism; Endoplasmic reticulum; Endoplasmic Reticulum Chaperone BiP; Endoplasmic Reticulum Stress; Endoribonucleases - metabolism; Epithelial cells; Epithelium; Failure; Gas exchange; Hepatitis; Homeostasis; Humans; In vivo methods and tests; Infections; Injuries; Inositol; Inositols; Kinases; Lavage; Lungs; Mice; Murine hepatitis virus - pathogenicity; Protein folding; Protein Serine-Threonine Kinases - metabolism; Proteins; Pulmonary Surfactants - metabolism; Respiration; Respiratory diseases; Respiratory failure; Respiratory Insufficiency - metabolism; Respiratory Insufficiency - pathology; Respiratory Insufficiency - virology; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Surface tension; Surfactants; Unfolded Protein Response; Viral diseases; Weight loss; alveolar type 2 cell; SARS-CoV-2; mouse hepatitis virus-1 (MHV-1); transitional epithelial cell state; pulmonary surfactant
• ISSN: 1040-0605; 1522-1504; 1522-1504
• DOI: 10.1152/ajplung.00324.2023

COVID-19 syndrome is characterized by acute lung injury, hypoxemic respiratory failure, and high mortality. Alveolar type 2 (AT2) cells are essential for gas exchange, repair, and regeneration of distal lung epithelium. We have shown that the causative agent, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and other members of the β-coronavirus genus induce an endoplasmic reticulum (ER) stress response in vitro; however, the consequences for host AT2 cell function in vivo are less understood. To study this, two murine models of coronavirus infection were used-mouse hepatitis virus-1 (MHV-1) in A/J mice and a mouse-adapted SARS-CoV-2 strain. MHV-1-infected mice exhibited dose-dependent weight loss with histological evidence of distal lung injury accompanied by elevated bronchoalveolar lavage fluid (BALF) cell counts and total protein. AT2 cells showed evidence of both viral infection and increased BIP/GRP78 expression, consistent with activation of the unfolded protein response (UPR). The AT2 UPR included increased inositol-requiring enzyme 1α (IRE1α) signaling and a biphasic response in PKR-like ER kinase (PERK) signaling accompanied by marked reductions in AT2 and BALF surfactant protein (SP-B and SP-C) content, increases in surfactant surface tension, and emergence of a reprogrammed epithelial cell population ( and ). The loss of a homeostatic AT2 cell state was attenuated by treatment with the IRE1α inhibitor OPK-711. As a proof-of-concept, C57BL6 mice infected with mouse-adapted SARS-CoV-2 demonstrated similar lung injury and evidence of disrupted surfactant homeostasis. We conclude that lung injury from β-coronavirus infection results from an aberrant host response, activating multiple AT2 UPR stress pathways, altering surfactant metabolism/function, and changing AT2 cell state, offering a mechanistic link between SARS-CoV-2 infection, AT2 cell biology, and acute respiratory failure. COVID-19 syndrome is characterized by hypoxemic respiratory failure and high mortality. In this report, we use two murine models to show that β-coronavirus infection produces acute lung injury, which results from an aberrant host response, activating multiple epithelial endoplasmic reticular stress pathways, disrupting pulmonary surfactant metabolism and function, and forcing emergence of an aberrant epithelial transition state. Our results offer a mechanistic link between SARS-CoV-2 infection, AT2 cell biology, and respiratory failure.