Quadruplex real-time quantitative PCR assay for the detection of pathogens related to late-onset ventilator-associated pneumonia

A quadruplex real-time (RT) qPCR assay for the detection and quantification in 4h of Staphylococcusaureus, Pseudomonasaeruginosa, Acinetobacterbaumannii and Stenotrophomonasmaltophilia directly from bronchoalveolar lavage specimens was developed. The specificity of the assay was 100% for all four sp...

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Published inJournal of microbiological methods Vol. 81; no. 3; pp. 232 - 234
Main Authors Rios-Licea, Merab M., Bosques, Francisco J., Arroliga, Alejandro C., Galindo-Galindo, Juan O., Garza-Gonzalez, Elvira
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.06.2010
Subjects
Acinetobacter baumannii
Alveoli
Pseudomonas aeruginosa
Real-time PCR
Staphylococcus aureus
Ventilator-associated pneumonia
Pseudomonas aeruginosa
Acinetobacter baumannii
Ventilator-associated pneumonia
Real-time PCR
Staphylococcus aureus
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Summary:A quadruplex real-time (RT) qPCR assay for the detection and quantification in 4h of Staphylococcusaureus, Pseudomonasaeruginosa, Acinetobacterbaumannii and Stenotrophomonasmaltophilia directly from bronchoalveolar lavage specimens was developed. The specificity of the assay was 100% for all four species.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2010.03.018