Hepatotoxicity induced by subacute exposure of Mancozeb and Arsenic in Wistar rats: Mitigating effect of quercetin and catechin

• Published in: Journal of Trace Elements and Minerals (Online) Vol. 10; p. 100199
• Main Authors: Yousuf, Rasia, Verma, Pawan Kumar, Sharma, Priyanka, Sood, Shilpa, Pankaj, Nrip K., Bhat, Zuhaib F.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2024; Elsevier
• Subjects: Arsenic; Catechin; Hepatotoxicity; Mancozeb; Oxidative damage; Quercetin; Mancozeb; Catechin; Arsenic; Oxidative damage; Hepatotoxicity; Quercetin
• ISSN: 2773-0506; 2773-0506
• DOI: 10.1016/j.jtemin.2024.100199

Widespread use of Mancozeb (MZ) fungicide in endemic areas having high levels of arsenic (As+3) in the groundwater is likely to cause toxicosis in humans and animals. Therefore, the present study aimed to determine the hepatotoxic effect of MZ and As+3 interaction in Wistar rats and mitigating potential of quercetin and catechin on such toxicity. Sixty adult rats were randomly divided into 10 groups of 6 animals each. Group I served as control and group II was exposed to MZ (800 mg kg-1, PO). Groups III, IV, and V were provided drinking water containing As+3 at the rate of 10, 50 and 100 µg L-1, respectively. Groups VI, VII, and VIII were given drinking water containing As+3 at 10, 50 and 100 µg L-1, respectively, along with MZ. Groups IX and X received drinking water contaminated with 100 µg L-1, As+3 and MZ along with quercetin or catechin (50 mg kg-1 each), respectively. Significant (p <0.05) alterations in hepatic biomarkers in blood, liver antioxidant status and hepatic histoarchitecture were observed in animals treated with individual toxicants in a dose-dependent manner, however, such toxicity-induced changes were more severe in co-exposed rats. The administration of quercetin or catechin significantly mitigated the dual toxicant administration-driven changes in biochemical markers of hepatic damage, hepatic antioxidant profile and liver histomorphology. Both quercetin and catechin proved beneficial in reducing toxicant-induced oxidation-mediated hepatic damage as demonstrated by alleviation in altered levels of determinants of hepatic biochemical and oxidative stress parameters in addition to restoration of toxicant-induced histological alterations of hepatic tissue.