The antinociceptive effect produced by intracerebroventricular injection of spantide in the mouse formalin test

• Published in: Japanese Journal of Pharmacology Vol. 58; no. suppl.1; p. 176
• Main Authors: Tan-No, Koichi, Sakurada, Tsukasa, Uchiumi, Hiroshi, Katsumata, Kazushige, Manome, Yoichi, Sakurada, Shinobu, Kisara, Kensuke
• Format: Journal Article
• Language: English
• Published: The Japanese Pharmacological Society 1992
• ISSN: 0021-5198; 1347-3506
• DOI: 10.1016/S0021-5198(19)49028-8

The antinociceptive effect of intracerebroventricularly (i.c.v.) administered 「D-Arg^1 D-Trp^7,9 , Leu^11 」 J-substance P (spantide), a frequently used tachykinin antagonist, was examined using the mouse formalin test. Licking of the injected hindpaw was recorded after the injection of formalin (20 μl, 2%). The licking behavior induced by 2% formalin solution had two peaks, 0-5 min (early phase) and 10-25 min (late phase). Spantide was administered i.c.v. 5 min before or 10 min after injection of 2% formalin solution in order to evaluate the effect of spantide on the formalin-induced nociceptive response. Spantide produced a dose-dependent antinociception during the early and late phase. The induced antinociception during the early phase was antagonized by pretreatment with naloxone, an opioid antagonist. An opioid binding study using 「^^3 H」-naloxone in the mouse brain membrane preparation revealed that spantide was able to inhibit 「^^3 H」-naloxone binding with the preparation. These results suggest that spantide-induced antinociception during the early phase may involve the opioid receptor in the mouse brain.