EFFECTS OF DIETARY COMMERCIAL YEAST GLUCAN ON INNATE IMMUNE RESPONSE, HEMATOLOGICAL PARAMETERS, INTESTINAL MICROBIOTA AND GROWTH PERFORMANCE OF WHITE FISH (Rutilus frisii kutum) FRY

• Published in: Ribarstvo Vol. 72; no. 4; pp. 156 - 163
• Main Authors: Rufchaie, Rudabeh, Hoseinifar, Seyed Hossein
• Format: Journal Article
• Language: English
• Published: 2014
• Subjects: Freshwater; Rutilus frisii; Caspian Sea
• ISSN: 1330-061X; 1848-0586
• DOI: 10.14798/72.4.772

This study investigates the effects of different levels of commercial yeast glucan (CYG) on innate immune response, hematological parameters, intestinal microbiota, growth performance and survival rate of white fish (kutum) fry (Rutilus frisii). Specimens (1.1 plus or minus 0.15 g) were randomly allocated into 15 tanks (100 L) at a density of 25 fish per tank and triplicate groups were fed either a basal control diet (0% CYG) or a basal diet supplemented with yeast (0.5, 1, 1.5 or 2% CYG) for 60 days. All dietary CYG levels significantly increased the innate immune responses (IgM levels and lysozyme activity) (P < 0.05) and the highest survival was observed in the 1 and 1.5% CYG group. Compared to the control group, CYG had no effects on RBC, Hct and Hb (P > 0.05), but WBC levels were significantly higher in CYG fed fish (P < 0.05). Microbiological assessment indicated that the viable culturable autochthonous and lactic acid bacteria (LAB) levels significantly elevated in 0.5 and 1% treatments. Our results indicated that low levels of dietary CYG (0.5 and 1 %) improved growth performance, body protein levels and survival rate compared to the control treatment (P < 0.05). These results indicate that CYG stimulates innate immune response, improves growth performance and modulates intestinal microbiota of kutum fry. Thus, we suggest that low level CYG may be used as a beneficial dietary supplement in kutum fry culture.Original Abstract: Ova studija istrazuje utjecaj razlicitih razina komercijalnog kvasca glukana (CYG) na imunoloski odgovor, hematoloske parametre, crijevnu mikrofloru, brzinu rasta i stopu prezivljavanja kod mladji bijele bodorke (Rutilus frisii). Uzorci mladji (1,1 plus or minus 0,15 g) su slucajnim izborom podijeljeni u 15 spremnika (100 L) pri gustoci od 25 riba po spremniku, a hranjeni su u triplikatu skupina sa osnovnom kontrolnom ishranom (0% CYG) ili osnovnom ishranom nadopunjenom kvascem (0,5, 1, 1,5, ili 2% CYG) 60 dana.U svim CYG prehrambenim razinama znacajno se povecao imunoloski odgovor (razine IgM i lizozim aktivnost) (P< 0,05), a najvece prezivljavanje zabiljezeno je u CYG skupini 1 i 1,5%. U usporedbi s kontrolnom skupinom, CYG nije imala utjecaj na RBC, Hct i Hb (P> 0,05), ali WBC razina bila je znatno visa u riba koje su hranjene s CYG (P <0,05).Mikrobioloska procjena ukazuje da su razine zivih kultura autohtonih i mlijecno kiselih bakterija (LAB) znacajno povisene u 0,5 i 1% tretmanu. Rezultati pokazuju da niska razina ishrane s CYG (0,5 i 1%) uzrokuje bolju performansu rasta, razinu tjelesnih proteina i stopu prezivljavanja u usporedbi s kontrolnom (P <0,05). Ovi rezultati pokazuju da CYG stimulira imunoloski odgovor, poboljsava performanse rasta i modulira crijevnu mikrofloru kod mladji bijele bodorke. Dakle, predlazemo da se niska razina CYG moze se koristiti kao koristan dodatak prehrani u proizvodnji mladji bijele bodorke.