Enhanced arsenic stress tolerance in landrace and improved rice cultivars through modulation of gibberellic acid (GA3) synthesis and antioxidant metabolism via phosphorus and silicon supplementation

• Published in: Plant stress (Amsterdam) Vol. 13; p. 100511
• Main Authors: Nazir, Faroza, Khatoon, Sayeda, Mahajan, Moksh, Kumari, Sarika, AlAjmi, Mohamed F, Rehman, Md Tabish, Khan, M. Iqbal R.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2024; Elsevier
• Subjects: Carbohydrate metabolism; Defense systems; Nutrients homeostasis; Oxidative stress tolerance; Nutrients homeostasis; Carbohydrate metabolism; Defense systems; Oxidative stress tolerance
• ISSN: 2667-064X; 2667-064X
• DOI: 10.1016/j.stress.2024.100511

•Both P and Si regulate GA production and influence plant growth under As stress.•Combined P and Si supplementation excelled in As stress amelioration than individual treatment.•PBZ (GA biosynthesis inhibitor) validated the growth-promoting effects of GA in As-stressed plants.•Landrace rice cultivar thrived well in its effect than improved rice cultivar. Arsenic (As), an environmental pollutant, imposes toxic impacts on plant health. In recent years, researchers have focused on comprehending the As-induced intrusion in growth and metabolic components. However, the interactions between phosphorus (P) and silicon (Si) remain elusive, specifically in rice (Oryza sativa) cultivars. Therefore, the present study investigated the effects of P and Si on the defense mechanisms, nutrients accumulation, carbohydrate metabolism, gibberellic acid (GA3) synthesis, and growth responses in Indica rice cultivars (landrace and improved) under As stress. With this focus, our study potentially indicated that As stress (150 µM) negatively impacted the underlying physiological and growth traits, with more pronounced effects on the improved cultivar compared to the landrace. However, P (30 mg kg−1 soil) and/ or Si (1 mM) treatments have prominently reduced oxidative stress with improved defense mechanisms and GA synthesis. Modulation of enzymatic defense system increased in both cultivars but more pronounced in landrace, including superoxide dismutase (+36.47%, +24.43%), ascorbate peroxidase (+95.11%, +78.26%), along with non-enzymatic antioxidants such as ascorbate (+29.43%, +21.48%), and reduced glutathione (+75.45%, +62.34%) concentrations, respectively. Notably, GA concentration (+23.78%, +16.48%) was substantially increased by the cumulative application of P and Si under As stress in both cultivars but more conspicuous in the landrace, respectively. Further, employing GA biosynthesis inhibitor, paclobutrazol (PBZ; 10 µM), substantiated the input of GA-mediated As tolerance, wherein, PBZ reversed the impacts of P and Si on the endogenous GA concentration and defense metabolism. In summation, landrace outperformed improved cultivar leading to As tolerance. This implies that the landrace could be used for future breeding programs permitting in-depth considerations of P and Si-mediated GA3 synthesis under As stress conditions. [Display omitted]