Antioxidant protection of a polysaccharide produced by Chaetomium globosum CGMCC 6882 on H2O2-challenged HepG2 cells

• Published in: Carbohydrate polymer technologies and applications Vol. 8; p. 100530
• Main Authors: Wang, Zichao, Zheng, Yi, Lu, Wenzhuo, Yang, Jinchu, Feng, Yingjie, Li, Zhitao, Li, Na, Yang, Yahui, Wang, Qi, An, Lemei
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.12.2024; Elsevier
• Subjects: Antioxidant activity; Chaetomium globosum CGMCC 6882; Polysaccharide; Submerged fermentation; Waste kitchen oil; Submerged fermentation; Waste kitchen oil; Chaetomium globosum CGMCC 6882; Polysaccharide; Antioxidant activity
• ISSN: 2666-8939; 2666-8939
• DOI: 10.1016/j.carpta.2024.100530

•A polysaccharide (CGP-WKO) was successfully produced by Chaetomium globosum CGMCC 6882 from waste kitchen oil.•Scavenging capacity of CGP-WKO against DPPH, ABTS, hydroxyl and superoxide radicals was in a dose-dependent manner.•CGP-WKO attenuated oxidative damage of HepG2 cells induced by H2O2 via regulating antioxidant-related enzymes.•CGP-WKO increased contents of SOD, CAT and GSH-Px, and reduced MDA content in H2O2-challenged HepG2 cells. Effective utilization of waste kitchen oil (WKO) is important to environmental protection and economic development. Presently, an endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide (CGP-WKO) was successfully produced through submerged fermentation with the sole carbon source of WKO. CGP-WKO had a yield of 1.53 ± 0.27 g/L and contained 95.85 % ± 3.02 % carbohydrate and 2.94 % ± 0.62 % protein. Structural feature analysis indicated that CGP-WKO contained glucose, glucosamine, mannose, rhamnose, galactose, fructose, and glucuronic acid in a molar ratio of 30.38: 1.34: 32.22: 9.68: 1.59: 0.62: 3.73. The weight-averaged molecular weight of CGP-WKO was 26.64 kDa, and its polydispersity was 1.48. Moreover, antioxidant capacity detection in vitro demonstrated that when the concentration of CGP-WKO was 1.0 mg/mL, its scavenging effects against 1,1-diphenyl-2-picrylhydrazyl; 2,2′-azinobis-di-(3-ethyl-benzothiazolin-6-sulfonic acid) diammonium salt; and hydroxyl and superoxide radicals were 45.89 % ± 1.89 %, 55.89 % ± 1.12 %, 29.02 % ± 2.61 %, and 52.76 % ± 2.12 %, respectively; and the IC50 values were 6.4612, 0.8888, 13.8585, and 0.9398 mg/mL, respectively. CGP-WKO increased the content of superoxide dismutase, catalase, and glutathione peroxidase and reduced malondialdehyde content in H2O2-challenged HepG2 cells. Overall, the present work indicated that CGP-WKO can be used as an antioxidant agent in the food, cosmetics, and pharmaceutical industries.