Identification of Key Functional Residues in the Active Site of Human β1,4-Galactosyltransferase 7

• Published in: The Journal of biological chemistry Vol. 285; no. 48; pp. 37342 - 37358
• Main Authors: Talhaoui, Ibtissam, Bui, Catherine, Oriol, Rafael, Mulliert, Guillermo, Gulberti, Sandrine, Netter, Patrick, Coughtrie, Michael W.H., Ouzzine, Mohamed, Fournel-Gigleux, Sylvie
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 26.11.2010; American Society for Biochemistry and Molecular Biology
• Subjects: Amino Acid; Enzyme Kinetics; Enzyme Mutation; Galactosyltransferase; Glycosaminoglycan; Life Sciences; Proteoglycan Synthesis; Xyloside; Amino Acid; Proteoglycan Synthesis; Glycosaminoglycan; Galactosyltransferase; Enzyme Mutation; Enzyme Kinetics; Xyloside
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M110.151951

Glycosaminoglycans (GAGs) play a central role in many pathophysiological events, and exogenous xyloside substrates of β1,4-galactosyltransferase 7 (β4GalT7), a major enzyme of GAG biosynthesis, have interesting biomedical applications. To predict functional peptide regions important for substrate binding and activity of human β4GalT7, we conducted a phylogenetic analysis of the β1,4-galactosyltransferase family and generated a molecular model using the x-ray structure of Drosophila β4GalT7-UDP as template. Two evolutionary conserved motifs, 163DVD165 and 221FWGWGREDDE230, are central in the organization of the enzyme active site. This model was challenged by systematic engineering of point mutations, combined with in vitro and ex vivo functional assays. Investigation of the kinetic properties of purified recombinant wild-type β4GalT7 and selected mutants identified Trp224 as a key residue governing both donor and acceptor substrate binding. Our results also suggested the involvement of the canonical carboxylate residue Asp228 acting as general base in the reaction catalyzed by human β4GalT7. Importantly, ex vivo functional tests demonstrated that regulation of GAG synthesis is highly responsive to modification of these key active site amino acids. Interestingly, engineering mutants at position 224 allowed us to modify the affinity and to modulate the specificity of human β4GalT7 toward UDP-sugars and xyloside acceptors. Furthermore, the W224H mutant was able to sustain decorin GAG chain substitution but not GAG synthesis from exogenously added xyloside. Altogether, this study provides novel insight into human β4GalT7 active site functional domains, allowing manipulation of this enzyme critical for the regulation of GAG synthesis. A better understanding of the mechanism underlying GAG assembly paves the way toward GAG-based therapeutics.