Xenopus Tadpole Tissue Harvest

• Published in: Cold Spring Harbor protocols Vol. 2017; no. 11; p. pdb.prot097675
• Main Authors: Patmann, Matthew D, Shewade, Leena H, Schneider, Katelin A, Buchholz, Daniel R
• Format: Journal Article
• Language: English
• Published: United States 01.11.2017
• Subjects: Animal Structures - anatomy & histology; Animals; Dissection - methods; Larva; Xenopus - embryology
• ISSN: 1559-6095
• DOI: 10.1101/pdb.prot097675

The procedures described here apply to tadpoles from the beginning of feeding through the major changes of metamorphosis and are appropriate for downstream postoperative snap freezing for molecular analysis, fixation for histological analysis, and sterile organ culture. To the uninitiated, the most difficult aspects of tadpole tissue dissections are likely knowing the appearance and location of organs, and the difficulty manipulating and holding tadpoles in place to carry out the oftentimes fine and precise dissections. Therefore, images and stepwise instructions are given for the harvest of external organs (tail, head, eyes, tail skin, back skin, gills, thymus, hind limbs, forelimbs) and peritoneal organs (intestine, pancreas, liver, spleen, lungs, fat bodies, kidney/gonad complex), as well as brain, heart, and blood. Dissections are typically done under a dissection stereomicroscope, and two pairs of fine straight forceps, one pair of fine curved forceps, and one pair of microdissection scissors are sufficient for most tissue harvests.