The determination of stigmastadienes in vegetable oils. Results of collaborative studies and the standardized method

The development by collaborative study of a standardised method for the determination of the stigmastadienes content in vegetable oils is described. The procedure involves the isolation of the unsaponifiable matter in the oil ands its fractioning on a silica gel column. The fraction containing the s...

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Bibliographic Details
Published inPure and applied chemistry Vol. 71; no. 2; pp. 349 - 359
Main Authors Dobarganes, M. C., Cert, A., Dieffenbacher, A.
Format Journal Article
LanguageEnglish
Published Berlin De Gruyter 28.02.1999
Walter de Gruyter GmbH
Subjects
Collaboration
Gas chromatography
Silica gel
Silicon dioxide
Vegetable oils
Vegetables
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Summary:The development by collaborative study of a standardised method for the determination of the stigmastadienes content in vegetable oils is described. The procedure involves the isolation of the unsaponifiable matter in the oil ands its fractioning on a silica gel column. The fraction containing the steroidal hydrocarbons is then analysed by capillary gas chromatography and the quantitative assessment made by the use of an internal standard /cholea-3,5-staddiene). The procedure is very sensitive allowing determination at levels of 0.01 - 4.0 mg/kg.
ISSN:0033-4545
1365-3075
DOI:10.1351/pac199971020349