The determination of stigmastadienes in vegetable oils. Results of collaborative studies and the standardized method
The development by collaborative study of a standardised method for the determination of the stigmastadienes content in vegetable oils is described. The procedure involves the isolation of the unsaponifiable matter in the oil ands its fractioning on a silica gel column. The fraction containing the s...
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Published in | Pure and applied chemistry Vol. 71; no. 2; pp. 349 - 359 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Berlin
De Gruyter
28.02.1999
Walter de Gruyter GmbH |
Subjects | |
Online Access | Get full text |
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Summary: | The development by collaborative study of a standardised method for the determination of the stigmastadienes content in vegetable oils is described. The procedure involves the isolation of the unsaponifiable matter in the oil ands its fractioning on a silica gel column. The fraction containing the steroidal hydrocarbons is then analysed by capillary gas chromatography and the quantitative assessment made by the use of an internal standard /cholea-3,5-staddiene). The procedure is very sensitive allowing determination at levels of 0.01 - 4.0 mg/kg. |
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ISSN: | 0033-4545 1365-3075 |
DOI: | 10.1351/pac199971020349 |