Expression of green fluorescent protein in Lactococcus lactis

The gfp gene from Aequorea victoria, encoding the green fluorescent protein (GFP) has been expressed in Lactococcus lactis subsp. lactis biovar cremoris MG1363, upon construction and introduction of plasmid pLS1GFP into this host. GFP was monitored in living cells during growth to evaluate its use i...

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Bibliographic Details
Published inFEMS microbiology letters Vol. 183; no. 2; pp. 229 - 234
Main Authors Fernández de Palencia, Pilar, Nieto, Concha, Acebo, Paloma, Espinosa, Manuel, López, Paloma
Format Journal Article
LanguageEnglish
Published Elsevier B.V 15.02.2000
Subjects
Aequorea victoria
Green fluorescent protein
Lactic acid bacterium
Lactococcus lactis
Milk
Plasmid pLS1
Lactococcus lactis
Plasmid pLS1
Lactic acid bacterium
Green fluorescent protein
Milk
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Summary:The gfp gene from Aequorea victoria, encoding the green fluorescent protein (GFP) has been expressed in Lactococcus lactis subsp. lactis biovar cremoris MG1363, upon construction and introduction of plasmid pLS1GFP into this host. GFP was monitored in living cells during growth to evaluate its use in molecular and physiological studies. Quantification of the levels of GFP expressed by cultures was feasible by fluorescence spectroscopy. Phase-contrast and fluorescence microscopy allowed us to distinguish, in mixed cultures, lactococcal cells expressing GFP. Our results indicate that GFP can be used as a reporter in L. lactis.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(99)00650-3