Enhancing vitamin D3 bioaccessibility: Unveiling hydrophobic interactions in soybean protein isolate and vitamin D3 binding via an infant in vitro digestion model

• Published in: Food chemistry Vol. 451; p. 139507
• Main Authors: Huang, Jia-Rong, Song, Jing-Ru, Cai, Wan-Shuang, Shao, Zhen-Wen, Zhou, Da-Yong, Song, Liang
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.09.2024
• Subjects: Dynamic-high-pressure microfluidization; Fortified yogurt; Hydrophobicity; Infant in vitro digestion; Vitamin D3 bioaccessibility; Fortified yogurt; Infant in vitro digestion; Hydrophobicity; Dynamic-high-pressure microfluidization; Vitamin D3 bioaccessibility
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2024.139507

In the domain of infant nutrition, optimizing the absorption of crucial nutrients such as vitamin D3 (VD3) is paramount. This study harnessed dynamic-high-pressure microfluidization (DHPM) on soybean protein isolate (SPI) to engineer SPI-VD3 nanoparticles for fortifying yogurt. Characterized by notable binding affinity (Ka = 0.166 × 105 L·mol−1) at 80 MPa and significant surface hydrophobicity (H0 = 3494), these nanoparticles demonstrated promising attributes through molecular simulations. During simulated infant digestion, the 80 MPa DHPM-treated nanoparticles showcased an impressive 74.4% VD3 bioaccessibility, delineating the pivotal roles of hydrophobicity, bioaccessibility, and micellization dynamics. Noteworthy was their traversal through the gastrointestinal tract, illuminating bile salts' crucial function in facilitating VD3 re-encapsulation, thereby mitigating crystallization and augmenting absorption. Moreover, DHPM treatment imparted enhancements in nanoparticle integrity and hydrophobic properties, consequently amplifying VD3 bioavailability. This investigation underscores the potential of SPI-VD3 nanoparticles in bolstering VD3 absorption, thereby furnishing invaluable insights for tailored infant nutrition formulations. [Display omitted] •Microfluidization enhanced SPI hydrophobicity, promoting bioactive interaction.•SPI's binding with VD3 regulated its release and transfer to bile salt micelles.•Improved VD3 mobility during digestion boosted its bioaccessibility.•Developed SPI-VD3 nanoparticles broaden applications in infant foods.