Nucleic Acid Probes in Microbiology

Single-stranded nucleic acid molecules can interact under certain conditions with other nucleic acids in the regions of base complementarily to form double-stranded hybrid molecules. From measurement of kinetics of the hybridisation and the extent of hybrid formation, information on nucleotide seque...

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Bibliographic Details
Published inDefense science journal Vol. 41; no. 4; pp. 335 - 356
Main Authors Venkateswaran, Neeraja, Venkateswaran, K.S.
Format Journal Article
LanguageEnglish
Published New Delhi Defence Scientific Information & Documentation Centre 01.01.1991
Subjects
Microbiology
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Summary:Single-stranded nucleic acid molecules can interact under certain conditions with other nucleic acids in the regions of base complementarily to form double-stranded hybrid molecules. From measurement of kinetics of the hybridisation and the extent of hybrid formation, information on nucleotide sequence heterogeneity of nucleic acid fractions, sequence properties of purified classes of molecules or the degree of homology between different samples of nucleic acids can be obtained. A specific deoxyribonucleic acid or ribonucleic acid sequence with an identifiable marker can be used as a nucleic acid probe. This paper reviews the use of nucleic acid probes for classifying the microorganisms and their detection in clinical or environmental samples.
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ISSN:0011-748X
0976-464X
DOI:10.14429/dsj.41.4436