DOPE–oleic acid–Ca2+ as DNA condensing agent

Phospholipid-based non-viral carriers composed of neutral phospholipid dioleoylphosphatidylethanolamine (DOPE) and the binary mixture DOPE–oleic acid (OA) are examined as potential DNA delivery vectors. The process of DNA condensation in the presence of Ca ions has been monitored through changes in...

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Bibliographic Details
Published inActa Facultatis Pharmaceuticae Universitatis Comenianae Vol. 65; no. 1; pp. 1 - 9
Main Authors Galliková, D., Liskayová, G., Búcsi, A., Hubčík, L., Martínez, J.C., Uhríková, D.
Format Journal Article
LanguageEnglish
Published Warsaw Sciendo 01.06.2018
De Gruyter Poland
Subjects
calcium
Deoxyribonucleic acid
DNA
DOPE
oleic acid
SAXD
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Summary:Phospholipid-based non-viral carriers composed of neutral phospholipid dioleoylphosphatidylethanolamine (DOPE) and the binary mixture DOPE–oleic acid (OA) are examined as potential DNA delivery vectors. The process of DNA condensation in the presence of Ca ions has been monitored through changes in emmision intensity of fluorescent probe ethidium bromide. The decline in fluorescence intensity with increasing Ca concentration at two different time intervals was correlated with the binding capacity of complexes and possible release of DNA from the complex. The microstructure of DOPE–OA mixtures at different OA/DOPE molar ratios and that of DOPE–OA–DNA–Ca complexes were determined using synchrotron small angle X-ray diffraction (SAXD). We identified inverted hexagonal phase H as the dominant structure. OA affects the lattice parameter of H formed by DOPE. With the increasing OA/DOPE molar ratio, the lattice parameter decreases, which results in significantly lower fraction of DNA bound to the OA-enriched complexes.
ISSN:2453-6725
1338-6786
2453-6725
DOI:10.2478/afpuc-2018-0001