Effect of anti-Ia antibodies, culture, and cyclosporin on prolongation of canine islet allograft survival
Effect of anti-Ia antibodies, culture, and cyclosporin on prolongation of canine islet allograft survival. R Alejandro , Z Latif , J Noel , F L Shienvold and D H Mintz Abstract Evidence in rodents suggests that islet pretreatment to reduce islet immunogenicity will also require some form of immunosu...
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Published in | Diabetes (New York, N.Y.) Vol. 36; no. 3; pp. 269 - 273 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Alexandria, VA
American Diabetes Association
01.03.1987
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Subjects | |
Online Access | Get full text |
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Summary: | Effect of anti-Ia antibodies, culture, and cyclosporin on prolongation of canine islet allograft survival.
R Alejandro ,
Z Latif ,
J Noel ,
F L Shienvold and
D H Mintz
Abstract
Evidence in rodents suggests that islet pretreatment to reduce islet immunogenicity will also require some form of immunosuppression
of the recipient for islet allograft acceptance in highly reactive donor-recipient pairs. We attempted to ascertain whether
outbred dogs would also require treatment of both donor islets and the recipient to prolong islet allograft survival. Untreated
canine islets are uniformly rejected in 6-10 days in beagles. Tissue culture alone, at 37 degrees C for 7 days, or treatment
of freshly prepared islets with anti-Ia monoclonal antibodies (MoAbs) (B1F6 + 7.2) did not prolong canine islet allograft
survival. Treatment of culture-maintained canine islets with anti-Ia MoAbs plus complement resulted in prolongation of islet
allograft survival for 188 and 368 days in two of seven pancreatectomized nonimmunosuppressed beagles. The administration
of low doses of cyclosporin A (CsA) intramuscularly, to recipients of untreated canine islet allografts had no effect on graft
survival. By contrast, six of nine CsA-treated recipients of islets that were also treated with anti-Ia MoAbs (B1F6 + 7.2)
plus complement showed prolongation of graft survival. Euglycemia was sustained for 19, 34, 89, and 300 days after the CsA
was discontinued (day 30) in four of these animals. Two animals had unstable grafts from the beginning that failed 23 and
29 days after transplantation. Our results indicate that simple maneuvers like short-term tissue culture at 37 degrees C and
treatment of freshly isolated islets with anti-Ia MoAbs and complement are inadequate to prevent rejection in outbred pancreatectomized
beagles. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0012-1797 1939-327X 0012-1797 |
DOI: | 10.2337/diabetes.36.3.269 |