Determination of Vitamin A ha Pharmaceutical Preparations by High-Speed Liquid Chromatography

In an attempt to develop a more specific and convenient method for the determination of vitamin A in preparations, high-speed liquid chromatographic method was investigated. Samples were dissolved, directly or after saponification, in a mixture of methanol and chloroform, and injected into a Permaph...

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Bibliographic Details
Published inYAKUGAKU ZASSHI Vol. 96; no. 5; pp. 669 - 672
Main Authors TAMEGAI, TOMOZOH, OHMAE, MASAHIKO, KAWABE, KIYOSHI
Format Journal Article
LanguageJapanese
English
Published The Pharmaceutical Society of Japan 25.05.1976
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Summary:In an attempt to develop a more specific and convenient method for the determination of vitamin A in preparations, high-speed liquid chromatographic method was investigated. Samples were dissolved, directly or after saponification, in a mixture of methanol and chloroform, and injected into a Permaphase "ODS" column. Vitamin A was separated from other substances by employing a mixture of methanol and water as the mobile phase, and assayed using chrysene or benzo[a]pyrene as internal standard. This method showed a substantial improvement over the Carr-Price method which had been used officially.
ISSN:0031-6903
1347-5231
DOI:10.1248/yakushi1947.96.5_669