Determination of Vitamin A ha Pharmaceutical Preparations by High-Speed Liquid Chromatography
In an attempt to develop a more specific and convenient method for the determination of vitamin A in preparations, high-speed liquid chromatographic method was investigated. Samples were dissolved, directly or after saponification, in a mixture of methanol and chloroform, and injected into a Permaph...
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Published in | YAKUGAKU ZASSHI Vol. 96; no. 5; pp. 669 - 672 |
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Main Authors | , , |
Format | Journal Article |
Language | Japanese English |
Published |
The Pharmaceutical Society of Japan
25.05.1976
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Online Access | Get full text |
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Summary: | In an attempt to develop a more specific and convenient method for the determination of vitamin A in preparations, high-speed liquid chromatographic method was investigated. Samples were dissolved, directly or after saponification, in a mixture of methanol and chloroform, and injected into a Permaphase "ODS" column. Vitamin A was separated from other substances by employing a mixture of methanol and water as the mobile phase, and assayed using chrysene or benzo[a]pyrene as internal standard. This method showed a substantial improvement over the Carr-Price method which had been used officially. |
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ISSN: | 0031-6903 1347-5231 |
DOI: | 10.1248/yakushi1947.96.5_669 |