Nutritional Variation of Human Tubercle Bacilli Growth on Glycerol-free Media

• Published in: Nihon saikingaku zasshi Vol. 19; no. 8; pp. 197 - 200
• Main Authors: HIRANO, Norimasa, SUSHIDA, Kiyo, NOJI, Kimi
• Format: Journal Article
• Language: Japanese
• Published: Japan JAPANESE SOCIETY FOR BACTERIOLOGY 1964
• Subjects: Agar; Animals; Biological Phenomena; Communications Media; Glycerol; Guinea Pigs; Humans; Mice; Mycobacterium tuberculosis; Old Medline; Pharmacology; Physiological Phenomena; GUINEA PIGS; PHARMACOLOGY; EXPERIMENTAL LAB STUDY; AGAR; MICE; GLYCERIN; MYCOBACTERIUM TUBERCULOSIS
• ISSN: 0021-4930; 1882-4110
• DOI: 10.3412/jsb.19.197

In preceding papers authors reported that human tubercle bacilli H 37 Rv, H2, and Aoyama B showed development on nutrient agar 4 weeks after incubation. This problem was studied in detail in this ex periment, and the following results were obtained. 1. When colonies of tubercle bacilli isolated on the Wgawa medium from sputum of patients wer subcultured on nutrient agar (the first subculture), some of the colonies grew on nutrient agar. Also after the second, third and fourth subculture on the Ogawa medium a few strains developed on nutrient agar and the remaining strains did not grow on the agar slant. 2. The colonies developed on nutrient agar were often so small that occasionally a hand lens had to be employed to recognize them, and almost all of the colonies showed development on the nutrient agar after about three or four weeks, but third or fourth subculture on nutrient agar often showed a rapid growth, so that their colonies were observed one week after seeding. 3. Tubercle bacilli developed on nutrient agar grew so well on the synthetic medium without glycerol that the pellicle covered the surface about one month after seeding.