Characterization of an Avian ( Gallus gallus domesticus ) TCR αδ Gene Locus

• Published in: The Journal of immunology (1950) Vol. 163; no. 7; pp. 3858 - 3866
• Main Authors: Kubota, Takayuki, Wang, Jin-yi, Göbel, Thomas W. F., Hockett, Richard D., Cooper, Max D., Chen, Chen-lo H.
• Format: Journal Article
• Language: English
• Published: 01.10.1999
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.163.7.3858

Abstract Mammalian TCRδ genes are located in the midst of the TCRα gene locus. In the chicken, one large Vδ gene family, two Dδ gene segments, two Jδ gene segments, and one Cδ gene have been identified. The TCRδ genes were deleted on both alleles in αβ T cell lines, thereby indicating conservation of the combined TCRαδ locus in birds. Vα and Vδ gene segments were found to rearrange with one, both or neither of the Dδ segments and either of the two Jδ segments. Exonuclease activity, P-addition, and N-addition during VDJδ rearrangement contributed to TCRδ repertoire diversification in the first embryonic wave of T cells. An unbiased Vδ1 repertoire was observed at all ages, but an acquired Jδ1 usage bias occurred in the TCRδ repertoire. The unrestricted combinatorial diversity of relatively complex TCRγ and δ loci may contribute to the remarkable abundance of γδ T cells in this avian representative.