Optimized [1- 13C]glucose infusion protocol for 13C magnetic resonance spectroscopy at 3 T of human brain glucose metabolism under euglycemic and hypoglycemic conditions

The effect of insulin-induced hypoglycemia on cerebral glucose metabolism is largely unknown. 13C MRS is a unique tool to study cerebral glucose metabolism, but the concurrent requirement for [1- 13C]glucose administration limits its use under hypoglycemic conditions. To facilitate 13C MRS data anal...

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Bibliographic Details
Published inJournal of neuroscience methods Vol. 186; no. 1; pp. 68 - 71
Main Authors van de Ven, Kim C.C., van der Graaf, Marinette, Tack, Cees J.J., Klomp, Dennis W.J., Heerschap, Arend, de Galan, Bastiaan E.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 2010
Subjects
13C
Brain glucose metabolism
Hypoglycemia
Magnetic resonance spectroscopy
Magnetic resonance spectroscopy
13C
Hypoglycemia
Brain glucose metabolism
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Summary:The effect of insulin-induced hypoglycemia on cerebral glucose metabolism is largely unknown. 13C MRS is a unique tool to study cerebral glucose metabolism, but the concurrent requirement for [1- 13C]glucose administration limits its use under hypoglycemic conditions. To facilitate 13C MRS data analysis we designed separate [1- 13C]glucose infusion protocols for hyperinsulinemic euglycemic and hypoglycemic clamps in such a way that plasma isotopic enrichment of glucose was stable and comparable under both glycemic conditions. 13C MR spectra were acquired with optimized 13C MRS measurement techniques to obtain high quality 13C MR spectra with these protocols.
ISSN:0165-0270
1872-678X
DOI:10.1016/j.jneumeth.2009.10.025