Isolation of a new laccase isoform from the white-rot fungi Pycnoporus cinnabarinus strain ss3

Two extracellular laccase isoforms (Lac I and Lac II) produced by the white-rot fungus Pycnoporus cinnabarinus from the monokaryotic strain ss3 were purified from ferulic-acid-induced liquid culture medium using ammonium sulphate precipitation, followed by anion-exchange chromatography on a Mono Q c...

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Bibliographic Details
Published inCanadian journal of microbiology Vol. 46; no. 8; pp. 759 - 763
Main Authors Otterbein, Ludovic, Record, Eric, Chereau, David, Herpoël, Isabelle, Asther, Marcel, Moukha, Serge M
Format Journal Article
LanguageEnglish
Published Ottawa, Canada NRC Research Press 01.08.2000
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Summary:Two extracellular laccase isoforms (Lac I and Lac II) produced by the white-rot fungus Pycnoporus cinnabarinus from the monokaryotic strain ss3 were purified from ferulic-acid-induced liquid culture medium using ammonium sulphate precipitation, followed by anion-exchange chromatography on a Mono Q column. Strain ss3 is the first generation of the parental strain P. cinnabarinus I-937. The new isolated isoform, Lac II, consists of an 86 000 molecular weight protein as determined by SDS polyacrylamide gel electrophoresis. The N-terminal amino acid sequences of both isoforms were determined, and compared to known laccase protein sequences of other organisms.Key words: oxydo-reductase, filamentous fungi, purification.
ISSN:0008-4166
1480-3275
DOI:10.1139/w00-039