Lipid-associated chlorophyll: evidence from 13C-NMR of the photosynthetic spinach thylakoid membrane
The 13C-NMR spectrum at 90.5 MHz has been obtained for the photosynthetic thylakoid membrane of spinach. Specific lipid and chlorophyll resonances can be assigned in the high resolution spectrum, although protein resonances are not observed. It can be estimated from resonance intensities that at lea...
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| Published in | Biochimica et biophysica acta Vol. 642; no. 2; p. 438 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Netherlands
06.04.1981
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0006-3002 |
| DOI | 10.1016/0005-2736(81)90460-0 |
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| Abstract | The 13C-NMR spectrum at 90.5 MHz has been obtained for the photosynthetic thylakoid membrane of spinach. Specific lipid and chlorophyll resonances can be assigned in the high resolution spectrum, although protein resonances are not observed. It can be estimated from resonance intensities that at least 30% of the plant chlorophyll contributes to the high resolution 13C spectrum with the remainder broadened by incomplete motional averaging. The resonance linewidths of the observed chlorophyll phytol chains are approximately the same as those of the lipid hydrocarbon chains, indicating a similar motional state and suggesting that this particular pool of chlorophyll is lipid-bound or at most only loosely associated with proteins. |
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| AbstractList | The 13C-NMR spectrum at 90.5 MHz has been obtained for the photosynthetic thylakoid membrane of spinach. Specific lipid and chlorophyll resonances can be assigned in the high resolution spectrum, although protein resonances are not observed. It can be estimated from resonance intensities that at least 30% of the plant chlorophyll contributes to the high resolution 13C spectrum with the remainder broadened by incomplete motional averaging. The resonance linewidths of the observed chlorophyll phytol chains are approximately the same as those of the lipid hydrocarbon chains, indicating a similar motional state and suggesting that this particular pool of chlorophyll is lipid-bound or at most only loosely associated with proteins. The 13C-NMR spectrum at 90.5 MHz has been obtained for the photosynthetic thylakoid membrane of spinach. Specific lipid and chlorophyll resonances can be assigned in the high resolution spectrum, although protein resonances are not observed. It can be estimated from resonance intensities that at least 30% of the plant chlorophyll contributes to the high resolution 13C spectrum with the remainder broadened by incomplete motional averaging. The resonance linewidths of the observed chlorophyll phytol chains are approximately the same as those of the lipid hydrocarbon chains, indicating a similar motional state and suggesting that this particular pool of chlorophyll is lipid-bound or at most only loosely associated with proteins.The 13C-NMR spectrum at 90.5 MHz has been obtained for the photosynthetic thylakoid membrane of spinach. Specific lipid and chlorophyll resonances can be assigned in the high resolution spectrum, although protein resonances are not observed. It can be estimated from resonance intensities that at least 30% of the plant chlorophyll contributes to the high resolution 13C spectrum with the remainder broadened by incomplete motional averaging. The resonance linewidths of the observed chlorophyll phytol chains are approximately the same as those of the lipid hydrocarbon chains, indicating a similar motional state and suggesting that this particular pool of chlorophyll is lipid-bound or at most only loosely associated with proteins. |
| Author | Chant, S I Croasmun, W R Eigenberg, K E |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/7284366$$D View this record in MEDLINE/PubMed |
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| Snippet | The 13C-NMR spectrum at 90.5 MHz has been obtained for the photosynthetic thylakoid membrane of spinach. Specific lipid and chlorophyll resonances can be... |
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| SubjectTerms | Chlorophyll - metabolism Chloroplasts - metabolism Intracellular Membranes - metabolism Magnetic Resonance Spectroscopy Membrane Lipids - metabolism Photosynthesis Vegetables |
| Title | Lipid-associated chlorophyll: evidence from 13C-NMR of the photosynthetic spinach thylakoid membrane |
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